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Morphokinetic selection of embryos (Time lapse)

In order to assess how the embryos develop in the process of cultivation and select the best ones for transfer and cryopreservation, the embryologist periodically takes them out from the incubator and inspects using the inverted microscope. This is a routine, but very important procedure in each embryology laboratory, in its course, the quality and rate of development of the embryo are estimated. However, even with a short-term removal of the embryos for inspection (usually less than a minute), the temperature and pH of the culture medium, in which the embryos are placed, are changed. In addition, in each inspection the embryos are exposed to uncontrolled light, weak magnetic fields and currents – all these factors can have a negative impact on their development.

Morphokinetic selection of embryos

The mother’s body well protects the eggs and embryos, and provides them with stable conditions for development. Outside the human body, they are very sensitive to fluctuations in environmental conditions, so it is critically important to bring the conditions for keeping the embryos close to the natural. For this purpose, in Academician V.I. Gryshchenko Clinic for Reproductive Medicine the advanced technology for continuous observation of embryos implemented, which allows carrying out inspection, assessment of the quality and rate of development of embryos, without opening the incubator and not taking them out. This became possible due to the developments of the company Cryo Innovation and the emergence of the system PrimoVision, allowing on a real-time basis carrying out digital photography directly in an incubator and thus monitor all stages of development of the embryos without changes in conditions of cultivation. The system is based on the use of innovative technology of time-lapse video, during which photographing of each embryo is carried out with a given frequency from the moment of fertilization and throughout the whole incubation period. It should be noted that when carrying out photography, led green light of low intensity is used, and for the period of 5-day stay in the incubator under monitoring by PrimoVision, total light load for the embryo is only about 10% of the obtained load under standard inspection alone under a conventional microscope. Thus, application of the new approach to monitoring the embryos inside incubators significantly improves the stability of conditions for their development.

But it’s not the only value of the time-lapse technology. In IVF cycles, the embryologist most often deals with a number of embryos. These embryos are different: some of them are perfect, others are less promising, and some are abnormal. One of the most important tasks facing the embryologist is selection of the most promising embryos for transfer into the uterine cavity. To this solve this task, the embryologist evaluates the appearance of embryos – studies certain morphological signs. For the whole period of cultivation (up to 120 hours) the embryologist can afford only a very limited number of inspections of embryos – usually 2–4 brief inspections. One could say that at the traditional method of evaluating the embryologist just had a few “shots” of the early life of the embryos, on the basis of which he/she should make selection of the best of them. During the rest of time, development of the embryo was hidden from the view of the embryologist.

 

With the advent of technology for continuous observation of embryos, embryologists get the most detailed video-chronicles of early development of the each individual embryo. In the course of its development, the embryo undergoes a series of key events (stages of development) and the time that the embryo passes from one stage of development to the next is an important indicator in evaluating the quality and capacity of implantation – the kinetics of development. Thus, studies of morphology and development of embryos allowed development of morphokinetic criteria to evaluate the quality of the embryos that significantly complements traditional approaches.

Due to the time-lapse technology in the armoury of embryologists a new tool has appeared, which allows improving selection of the most promising embryos and thus to increase the probability of pregnancy.

    Cost of services "Morphokinetic selection of embryos (Time lapse)"

    Морфокинетическая селекция эмбрионов (0-9/9-18/19-27) 2100/2850/3500 UAH

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